il 1 β  (Boster Bio)

Journal: Acta Pharmaceutica Sinica. B

Article Title: Palmitoylation of Tfr1 enhances platelet ferroptosis and liver injury in heat stroke

doi: 10.1016/j.apsb.2025.10.027

Figure Lengend Snippet: Platelets participate in liver injury in the HS murine model. (A) Thermoregulatory graphical description of heat stress profile aspects within 28 h in mice (shown as mean only, total n = 25). (B) Platelet counts along the time axis at sham heat rest (SHR) and 0, 6, 24 h post-HS ( n = 6 mice per group). (C) Cell cytotoxicity of mouse platelets was measured by LDH activity under heat stress in vitro ( n = 6 mice per group). (D–F) Plasma ALT (D) and AST (E) and IL-1 β (F) levels in SHR mice and mice at 0, 6, 24 h post-HS ( n = 6 mice per group). (G–I) Correlation between platelet counts and Tc max at HS onset ( n = 16), ALT ( n = 24), and IL-1 β ( n = 24) levels. (J–L) Blood platelet counts (J), plasma ALT levels (K), and representative HE staining images of liver tissue (L) were assessed in SHR mice and mice at 0, 6, 24 h post-HS, with or without pretreatment with the platelet-depleting antibody α CD42b or the platelet stimulator thrombopoietin (TPO) ( n = 6 mice per group, scale bars: 100 μm). Mean ± SEM are shown, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

Article Snippet: Coagulative factors, interleukin-1 β (IL-1 β ) and lipid peroxidation metabolites were measured by enzyme-linked immunosorbent assay in accordance with the manufacturer's instructions, respectively: IL-1 β (CSB-E08054m/E08053h, CUSABIO, Wuhan, China), IL-6 (CSB-E04639m, CUSABIO), IL-10 (CSB-E04594m-IS, CUSABIO), TNF- α (CSB-E04741m, CUSABIO) and 4-hydroxynonenal (4-HNE, CSB-E13412m/E16214h, CUSABIO).

Techniques: Activity Assay, In Vitro, Clinical Proteomics, Staining

Journal: Acta Pharmaceutica Sinica. B

Article Title: Palmitoylation of Tfr1 enhances platelet ferroptosis and liver injury in heat stroke

doi: 10.1016/j.apsb.2025.10.027

Figure Lengend Snippet: Platelet-derived IL-1 β contributes to liver injury in the HS murine model. (A) GO (BP) analysis of the physiological and pathological states of platelets in SHR mice and mice at 0 h post-HS (HS0). (B) Representative images of immunofluorescence in liver tissue from SHR and HS mice ( n = 3 independent experiments, scale bars, 50 μm). DAPI (blue), CD42b for platelets (green), Clec4f for Kupffer cells (red), Glutamine Synthetase (GS) for central vein zone (purple). (C) Flow cytometric analysis of the percentage of P-selectin expression on platelets in SHR mice and mice at 0, 6, 24 h post-HS ( n = 6 mice per group). (D) GO (BP) analysis of cytokine-related pathways in platelets from SHR mice and at HS onset (HS0). (E, F) qPCR analysis of Il1b (E) and Casp1 (F) mRNA expression of platelets under heat stress in vitro ( n = 6 mice per group). (G) Flow cytometry analysis of caspase-1 levels in platelets from Tfr1 flox/flox mice, CKO- Tfr1 mice and CKO- Tfr1 mice infected with AAV9-DIO- Tfr1 , with or without ferroptosis inhibitor fer1 and DFO ( n = 6 mice per group). (H) ELISA analysis of IL-1 β levels in the supernatant of platelets under heat stress in vitro ( n = 6 mice per group). (I) ELISA analysis of IL-1 β levels in plasma from Tfr1 flox/flox mice, CKO- Tfr1 mice, and CKO- Tfr1 mice infected with AAV9-DIO- Tfr1 , with or without ferroptosis inhibitor fer1 and DFO ( n = 6 mice per group). (J) ELISA analysis of IL-1 β levels in PLP supernatant from sh- Zdhhc2, oe- Zdhhc2 , oe- Tfr1 , oe- Tfr1 -sh- Zdhhc2 MEG-01 cells under heat stress in vitro , with or without ferroptosis inhibitor fer1 ( n = 6 independent experiments). Mean ± SEM are shown, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.

Article Snippet: Coagulative factors, interleukin-1 β (IL-1 β ) and lipid peroxidation metabolites were measured by enzyme-linked immunosorbent assay in accordance with the manufacturer's instructions, respectively: IL-1 β (CSB-E08054m/E08053h, CUSABIO, Wuhan, China), IL-6 (CSB-E04639m, CUSABIO), IL-10 (CSB-E04594m-IS, CUSABIO), TNF- α (CSB-E04741m, CUSABIO) and 4-hydroxynonenal (4-HNE, CSB-E13412m/E16214h, CUSABIO).

Techniques: Derivative Assay, Immunofluorescence, Expressing, In Vitro, Flow Cytometry, Infection, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

Journal: Acta Pharmaceutica Sinica. B

Article Title: Palmitoylation of Tfr1 enhances platelet ferroptosis and liver injury in heat stroke

doi: 10.1016/j.apsb.2025.10.027

Figure Lengend Snippet: Tfr1 palmitoylation-mediated ferroptosis in platelets, accompanied by IL-1 β secretion, exacerbates liver injury in HS. The findings provide novel insights into thrombocytopenia and liver injury in HS, offering a potential therapeutic strategy to mitigate HS-induced thrombocytopenia, liver injury, and poor prognosis.

Article Snippet: Coagulative factors, interleukin-1 β (IL-1 β ) and lipid peroxidation metabolites were measured by enzyme-linked immunosorbent assay in accordance with the manufacturer's instructions, respectively: IL-1 β (CSB-E08054m/E08053h, CUSABIO, Wuhan, China), IL-6 (CSB-E04639m, CUSABIO), IL-10 (CSB-E04594m-IS, CUSABIO), TNF- α (CSB-E04741m, CUSABIO) and 4-hydroxynonenal (4-HNE, CSB-E13412m/E16214h, CUSABIO).

Techniques: